A simple method for extraction and quantitation of 14-C-labeled compounds from blood.
نویسندگان
چکیده
IJABELEI) URIC ACTI) (as a 0.50 c./ml. solution in dilute fornmalin) and glucose (as a 5.0 .tc./ml. solution in saturated henzoic acid) were used. Tlmese were diluted with distilled water, and 0.20-mi. aliquots were placed in the bottom of 25-mm. test tui)es om25-nil. Erlenmeyer flasks and evaporated to dryimess. Dilutions were calculated to keep counting rates between 4000 and 2 ,000 cprn. As previously described (1-4), 0.i0-nmi. aliquots of sei-unm (micro
منابع مشابه
A Simple and Sensitive HPLC Method for Fluorescence Quantitation of Doxorubicin in Micro-volume Plasma: Applications to Pharmacokinetic Studies in Rats
A validated HPLC method was developed to determine doxorubicin concentration in a small volume of rat plasma (60 µl) with convenient fluorescence detection. Sample preparation includes a simple one-step liquid-liquid extraction using minimum amount of organic solvent, with extraction recoveries more than 95%. The analysis was accomplished using PerfectSil C18 column maintained at 35 °C and a mo...
متن کاملA Simple and Sensitive HPLC Method for Fluorescence Quantitation of Doxorubicin in Micro-volume Plasma: Applications to Pharmacokinetic Studies in Rats
A validated HPLC method was developed to determine doxorubicin concentration in a small volume of rat plasma (60 µl) with convenient fluorescence detection. Sample preparation includes a simple one-step liquid-liquid extraction using minimum amount of organic solvent, with extraction recoveries more than 95%. The analysis was accomplished using PerfectSil C18 column maintained at 35 °C and a mo...
متن کاملA Rapid and Sensitive Method for Determina-tion of Morphine in Saliva of Opium Addicts
Background: Saliva is an alternative biological matrix in drug testing. It has several advantages in comparison with other biological fluids such as blood and urine. Collection of saliva is simple, noninvasive and can be controlled under direct observation in drug abusers, or opium addicts. Methods: In the present study we report our experience with saliva collection, liquid-liquid extraction a...
متن کاملEvaluation of a modified salt-out method for DNA extraction from whole blood lymphocytes: A simple and economical method for gene polymorphism
Extraction of high-quality and-quantity DNA is a fundamental requirement for genetic research. It is very important to address the use of DNA extraction methods that are simple and cost-effective in gene polymorphism with large number of samples. This study was designed to investigate the optimal DNA extraction from lymphocytic cells by salt-out method. In this study, 200 blood samples of the t...
متن کاملSimple and sensitive high performance liquid chromatographic method for the simultaneous quantitation of the phenylalanine in human plasma
Phenylalanine (Phe) is the most reliable indicator for the diagnosis of phenylketonuria (PKU). The purpose of this study is to establish a reliable and quick method for the assignment of Phe in peripheral capillary blood from newborns and children by high performance liquid chromatography with ultraviolet detection (HPLC-UV). PKU is an inborn error of metabolism characterized by the inability o...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 12 9 شماره
صفحات -
تاریخ انتشار 1966